Coding

Part:BBa_K3581013:Design

Designed by: HUI Wan Hei   Group: iGEM20_HKUST   (2020-10-13)


amilGFP yellow chromoprotein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Acc65I and AscI cut sites flank the coding region so to allow insertion of chromoprotein in N or C terminus of the protein.


Source

Original sequence from iGEM11 Uppsala Sweden.

References

1. Liljeruhm, J., Funk, S. K., Tietscher, S., Edlund, A. D., Jamal, S., Wistrand-Yuen, P., Dyrhage, K., Gynnå, A., Ivermark, K., Lövgren, J., Törnblom, V., Virtanen, A., Lundin, E. R., Wistrand-Yuen, E., & Forster, A. C. (2018). Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Journal of biological engineering, 12, 8. https://doi.org/10.1186/s13036-018-0100-0

2. https://parts.igem.org/Part:BBa_K592010